Synthetic mRNA

Messenger RNA (“mRNA”) is a special class of molecules containing instructions that determine how cells function. Brooklyn’s platform is being designed to harness mRNA to engineer cells to treat disease by repairing disease-causing mutations and directing the formation of stem cells.

Expressing reprogramming proteins by repeated transfection with protein-encoding RNA could avoid the limitations of both DNA and protein-based reprogramming techniques. However, transfection with long, in vitro-transcribed RNA triggers a potent innate immune response in human cells, even when the RNA is capped and polyadenylated to mimic eukaryotic mRNA. To address this problem, researchers have discovered methods of suppressing innate immunity, which enable frequent transfection with protein-encoding RNA.

Non-Viral Nucleic Acid Delivery

Historically, viruses were the primary method of delivering nucleic acids to cells and spawned the field of gene therapy. More recently, scientists have used non-viral delivery systems to enhance the uptake of nucleic acids by cells. However, conventional delivery systems often suffer from endosomal entrapment and toxicity, limiting their therapeutic use.

Brooklyn is using a novel chemical substance that is exceptionally effective in delivering nucleic acids, including mRNA, to cells both ex vivo and in vivo.

Cellular Reprogramming

Cellular reprogramming refers to the process of transforming patient skin cells into pluripotent stem cells, called induced pluripotent stem cells (iPSC). Since 2012 Nobel Prize Winner Dr. Shinya Yamanaka's initial discovery of iPSC in 2006, iPSC availability has transformed the field of cell therapy.

Brooklyn uses the fastest, highest-efficiency method for generating iPSC – a key invention now recognized by numerous patents. The patented methods can transform patient skin cells into stem cells more rapidly, efficiently, and safely than previous techniques.

Gene Editing

Our partners at Factor Bioscience have developed a technology that uses mRNA to express gene-editing proteins. This technology can enable dramatically higher efficiency gene editing, including in primary cells, than other approaches, without using viruses or DNA-based vectors that may cause unwanted mutagenesis.

We can use this technology to inactivate one or more genes and/or insert a donor sequence into a genomic safe harbor locus, enabling controlled expression of an exogenous gene.